Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the NIH or NSERC.Ĭompeting interests: The authors have declared that no competing interests exist. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. RK, DJH, and NLW were each supported by Biomedical Research Awards from the University of Saskatchewan. NSERC also provided support to EGH (Undergraduate Research Award) and MBP (Integrated Training Program in Infectious Diseases). JAP and KDM were supported by fellowships from the Saskatchewan Health Research Foundation.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: All relevant data are within the manuscript and its Supporting Information files.įunding: This work was supported by NIH grants AI137541, AI125429, AI126133, AI132996, AI148770, AI153325 and AI151893 to CT, by a Discovery Grant from the Natural Sciences and Engineering Research Council of Canada (NSERC #2017-05737) and the Jarislowsky Chair in Biotechnology to APW. Received: JanuAccepted: Published: July 9, 2020Ĭopyright: © 2020 Miller et al. PLoS Pathog 16(7):Įditor: Leigh Knodler, Washington State University, UNITED STATES (2020) In vivo synthesis of bacterial amyloid curli contributes to joint inflammation during S. Finally, our work provides conceptual evidence for the possibility of cross-seeding between bacterial amyloids like curli and human amyloids involved in amyloid-associated diseases such as Alzheimer’s Disease via the gut microbiome or infections.Ĭitation: Miller AL, Pasternak JA, Medeiros NJ, Nicastro LK, Tursi SA, Hansen EG, et al. As curli or curli-like amyloids are also produced by the commensal bacteria, it is possible that the unintended release of amyloids produced by the microbiota could trigger similar autoimmune reactions. Reactive arthritis is a known autoimmune complication after enteric infections and our results indicate that presence of curli in the gut provides a novel linchpin of pathogenesis. This finding is significant because of the immune impacts on the host we show that curli cause an increase in autoimmunity and inflammation in the knee joints of infected mice.

We present the first biochemical evidence that these fibers are produced in the gastrointestinal tract of mice after oral infection, the natural route for Salmonella infections. Our manuscript focuses on curli, a ‘functional amyloid’ produced by Salmonella as well as other enteric bacteria.

James patterson i even funnier